RNA in situ hybridization of dissected gonads.
نویسندگان
چکیده
Copyright: © 2006 Min-Ho Lee and Tim Schedl. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. To whom correspondence should be addressed. E-mail: [email protected] or [email protected] RNA in situ hybridization of dissected gonads* Min-Ho Lee, Tim Schedl, Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110 USA
منابع مشابه
دورگه‌سازی در محل؛ اصول و کاربردها : مقاله مروری
In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
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عنوان ژورنال:
- WormBook : the online review of C. elegans biology
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تاریخ انتشار 2006